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Eur Respir J 2006, doi:10.1183/09031936.00151905
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ORIGINAL ARTICLE |
Saliva is one likely source of leukotriene B4 in exhaled breath condensate
1 Division of Physiology, the National Institute of Environmental Medicine, Karolinska Institutet, SE-17177 Stockholm, Sweden
* To whom correspondence should be addressed. E-mail: Flora.Gaber{at}ki.se.
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Abstract |
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Leukotriene B4 (LTB4) in exhaled breath condensate (EBC) has been reported to be elevated in airway inflammation. The origin of leukotrienes in EBC is however not established.
To measure LTB4 levels in EBC collected in two challenges characterized by a strong neutrophilic airway inflammation. To compare LTB4 levels in EBC with levels in sputum and saliva.
Leukotriene B4 and 
-amylase were measured in EBC from 34 healthy subjects, exposed in a swine confinement building or to a lipopolysaccharide provocation. These markers were also measured in induced sputum in 11 of the subjects. For comparison, LTB4 and -amylase were measured in saliva from healthy subjects.
Only four out of 102 EBC samples had detectable LTB4 (28-100 pg·mL-1). Alpha-amylase activity was detected in the LTB4 positive samples. In contrast, LTB4 was detected in all examined sputum supernatants in the same study (median 1190 pg·mL-1). The median LTB4 level in saliva was 469 pg·mL-1.
High levels of LTB4 in saliva, and presence of LTB4 in EBC only when -amylase was detected, indicates that LTB4 found in EBC is due to saliva contamination. As LTB4 consistently was present in sputum supernatants, EBC may be inappropriate for monitoring airway LTB4.
-amylase, exhaled breath condensate, induced sputum, leukotriene B4, neutrophilic inflammation, pig house
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