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Published online before print September 5, 2007
Eur Respir J 2007, doi:10.1183/09031936.00053607
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ORIGINAL ARTICLE

Experimental pseudomonas aeruginosa pneumonia. evaluation of the associated inflammatory response

O. Sibila 1, C. Agustí 1, A. Torres 1, S. Baquero 2, S. Gando 2, J.R. Patrón 3, J.G. Morato 3, D.H. Goffredo 2, N. Bassi 4, C.M. Luna 2

1 Servei de Pneumologia. Institut Clínic del Tòrax. Hospital Clínic de Barcelona. IDIBAPS. Universitat de Barcelona, Spain
2 División Neumonología
3 Centro Universitario de Cirugía Experimental
4 División Patología, Hospital de Clínicas, Universidad de Buenos Aires, Argentina


  Abstract

An abnormal inflammatory response (IR) in pneumonia is associated with poor outcomes and high mortality. Animal models could help to better understand the relationship between the pulmonary infection and the associated IR.

The aim of the study was to validate an experimental model of pneumonia induced by the inoculation of Pseudomonas aeruginosa in ventilated piglets and to study the associated IR over a long period of time (96 h).

Five Lagerwhite-Landrace piglets were ventilated for four days. After intubation, a solution containing 75 ml of P. aeruginosa (106 cfu·ml-1) was bronchoscopically inoculated. Physiologic and laboratory parameters were monitored throughout the study. Proinflammatory cytokines were measured in serum and in bronchoalveolar lavage (BAL). Histopathology of the lungs and cultures from blood, BAL and lungs were performed.

All the animals developed histopathological evidence of pneumonia. Microbiological studies of both BAL and lung confirmed the presence of P. aeruginosa in all the samples. Throughout the study, an increase in IL-6 was observed in serum and in BAL.

In conclusion, this experimental model of pneumonia induced by the inoculation of high concentrations of P. aeruginosa in ventilated piglets is feasible and could be appropriate for the evaluation of different aspects of the associated IR.

Keywords:  Experimental pneumonia, IL-6, inflammatory response, Pseudomonas aeruginosa, ventilated piglets




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Copyright © 2007 by the European Respiratory Society.