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Increasing level of CD56+ T-cells in peripheral blood in sarcoidosis

¹ Service de Pneumologie, ² Laboratoire d'Anatomie pathologique, and, ³ Laboratoire de Biochimie et de Biologie moléculaire, Hôpital Avicenne, and, ⁴ Université Paris 13, UPRES-EA 3406, Bobigny, and ⁵ Laboratoire d'Hématologie et d'Immunologie Biologiques, Cytogénétique, INSERM E109, CHU Bicêtre, AP-HP, Paris, France.

To the Editors:

We read with interest the recent paper by Katchar et al. 1 reporting the characterisation of natural killer (NK) cells and CD56+ T-cells in sarcoidosis patients. The authors observed that, in the lung of patients with pulmonary sarcoidosis, NK cells have a distinct phenotype. They produce cytokines and participate actively in the T-helper (Th)-1-like inflammatory response. In addition, although they did not find any difference in the NK cell number between sarcoidosis patients and control subjects, the authors found an elevation of CD56+ T-cells in the peripheral blood (PB) of sarcoidosis patients. CD56+ T-cells are large granular lymphocytes with a significant proportion of CD4-CD8- cells or T-cells, and, to our knowledge, this is the first study in which such expansion of CD56+ T-cells in PB is described in sarcoidosis.

In a recent prospective study, we analysed the NK cells and CD56+ T-cells content in PB from 32 patients with pulmonary sarcoidosis and from 18 healthy volunteers by flow cytometry.

In sarcoidosis patients, we found that the lymphocyte count was lower (1,312±583·mm^–3) than in control subjects (2,439±481·mm^–3; p<0.0005), the NK cell count was identical (215±139·mm^–3 versus 281±144·mm^–3; p = 0.1) although the percentage was significantly different (18±10% versus 11±5%; p = 0.004), and finally both the CD56+ T-cell count and percentage were higher in sarcoidosis patients than in control subjects (158±122·mm^–3, 12±8% versus 53±45·mm^–3, 2±1%; p<0.0001).

With respect to the upper limit of normal CD56+ T-cell count (mean + 2SD), the patients could be separated in two groups: 1) group A (n = 15) comprising patients with elevated CD56+ T-cell counts (269±97·mm^–3); and 2) group B (n = 17) comprising patients with a normal CD56+ T-cell count (63·mm^–3). Interestingly, the patients in group B had a lower lymphocyte count (964±331·mm^–3) than the patients of group A (1,527±388·mm^–3; p = 0.0002) and a lower CD8+ T-cell count (173±102·mm^–3 versus 436±165·mm^–3; p<0.0001). However, these parameters did not correlate with the physical characteristics (i.e. sex and age), the clinical presentation (disease activity and radiographic staging) and the evolutionary trend of patients of the two groups.

CD56+ T-cells are cytotoxic cells that play an important role in the Th-1 responses 2. Their expansion is observed in the elderly and in pathologies involving chronic activation of the immune system, such as viral infections, rheumatic and autoimmune diseases or tumours 3. For instance, recently, Behcet's uveitis was found to be associated with an elevation of CD56+ T-cells in PB, which returned to normal after treatment 4, 5. Yet, in sarcoidosis, the functional significance of increased CD56+ T-cells is unclear.

Therefore, our results indicate that the polymorphism of the immune response in sarcoidosis and the clinical presentation are independent, underlining the fact that the status of peripheral CD56+ T-cells does not reflect the activity of sarcoidosis. However, together with the findings of Katchar et al. 1, our results indicate that studying the phenotypical, functional and cytokine production changes of these cells in peripheral blood (and in the sarcoid granuloma), before and after treatment, will be extremely informative.

REFERENCES

1. Katchar K, Soderstrom K, Wahlstrom J, Eklund A, Grunewald J. Characterisation of natural killer cells and CD56+ T-cells in sarcoidosis patients. Eur Respir J 2005;26:77–85.[Abstract/Free Full Text]
2. Ohkawa T, Seki S, Dobashi H, et al. Systematic characterization of human CD8+ T cells with natural killer cell markers in comparison with natural killer cells and normal CD8+ T cells. Immunology 2001;103:281–290.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
3. Tarazona R, DelaRosa O, Alonso C, et al. Increased expression of NK cell markers on T lymphocytes in aging and chronic activation of the immune system reflects the accumulation of effector/senescent T cells. Mech Ageing Dev 2000;121:77–88.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
4. Yato H, Matsumoto Y. CD56+ T cells in the peripheral blood of uveitis patients. Br J Ophthalmol 1999;83:1386–1388.[Abstract/Free Full Text]
5. Ahn JK, Seo JM, Yu J, Oh FS, Chung H, Yu HG. Down-regulation of IFN-gamma-producing CD56+ T Cells after combined low-dose cyclosporine/prednisone treatment in patients with Behcet's uveitis. Invest Ophthalmol Vis Sci 2005;46:2458–2464.[Abstract/Free Full Text]

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