ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Permissions
Right arrowRequest Permissions
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Balkom, R.
Right arrow Articles by Dekhuijzen, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Balkom, R.
Right arrow Articles by Dekhuijzen, P.
Eur Respir J 1996; 9: 1217-1223
Copyright © ERS Journals Ltd 1996

Original Articles

Effects of different treatment regimens of methylprednisolone on rat diaphragm contractility, immunohistochemistry and biochemistry

RH van Balkom, HF van der Heijden, HT van Moerkerk, JH Veerkamp, JA Fransen, LA Ginsel, HT Folgering, CL van Herwaarden, and PN Dekhuijzen

Systemic corticosteroid therapy may affect diaphragm structure and function. We postulated that functional, immunohistochemical and biochemical characteristics of rat diaphragm were less affected by alternate-day methylprednisolone (MP) administration, and more by repeated bursts of MP, in comparison to daily s.c MP. Sixty adult rats were randomized into four groups: saline s.c.; MP continuously (MP-C), 1 mg.kg-1 daily, MP alternate-day therapy (MP-A), 2 mg.kg-1 every other day; or MP in bursts (MP-B), MP 2 mg.kg-1 daily for 2 weeks, saline for 4 weeks, MP 2 mg.kg-1 daily for 2 weeks. The total treatment period was 8 weeks. Contractile properties of isolated diaphragm strips were measured. Antibodies reactive with type I, IIa, IIx and IIb myosin heavy chains were used for immunohistochemical analysis. Biochemical evaluation included markers of fast energy supply, glycogenolytic activity, beta-oxidation capacity and oxidative capacity. The force-frequency curve was depressed in all MP groups. Fibre type I, IIx and IIb cross-sectional area (CSA) decreased in all MP groups. Burst therapy decreased the contribution of type IIb fibres to total diaphragm muscle area. MP-A affected glycogenolytic activity less than MP-C. Burst MP therapy reduced creatine kinase (CK) activity and beta-oxidation capacity compared to MP-C. Oxidative capacity was increased in all MP groups. In conclusion, although the methylprednisolone treatment regimens affected diaphragm muscle morphology and bioenergetic enzyme activities in different ways, force generation decreased in all methylprednisolone treated groups to the same extent.


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. J. Verheul, C. B. Mantilla, W.-Z. Zhan, M. Bernal, P. N. R. Dekhuijzen, and G. C. Sieck
Influence of corticosteroids on myonuclear domain size in the rat diaphragm muscle
J Appl Physiol, November 1, 2004; 97(5): 1715 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
M. A. Pellegrino, G. D'Antona, S. Bortolotto, F. Boschi, O. Pastoris, R. Bottinelli, B. Polla, and C. Reggiani
Clenbuterol antagonizes glucocorticoid-induced atrophy and fibre type transformation in mice
Exp Physiol, January 1, 2004; 89(1): 89 - 100.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease . A Statement of the American Thoracic Society and European Respiratory Society
Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): S2 - 40.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. H. H. Van Balkom, P. N. R. Dekhuijzen, H. T. M. Folgering, J. H. Veerkamp, H. T. Van Moerkerk, J. A. M. Fransen, and C. L. A. Van Herwaarden
Anabolic steroids in part reverse glucocorticoid-induced alterations in rat diaphragm
J Appl Physiol, May 1, 1998; 84(5): 1492 - 1499.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. H. H. Van Balkom, W.-Z. Zhan, Y. S. Prakash, P. N. R. Dekhuijzen, and G. C. Sieck
Corticosteroid effects on isotonic contractile properties of rat diaphragm muscle
J Appl Physiol, October 1, 1997; 83(4): 1062 - 1067.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the European Respiratory Society.