ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murayama, T
Right arrow Articles by Kuze, F
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murayama, T
Right arrow Articles by Kuze, F
Eur Respir J 1996; 9: 293-300
Copyright © ERS Journals Ltd 1996


Original Articles

Suppressive effects of Aspergillus fumigatus culture filtrates on human alveolar macrophages and polymorphonuclear leucocytes

T Murayama, R Amitani, Y Ikegami, R Nawada, WJ Lee, and F Kuze

Aspergillus spp., especially A. fumigatus (Af) can colonize the airways and the lungs with localized underlying conditions and further invade the surrounding lung tissues, even in subjects without systemic predisposing factors, presumably by escaping the local host defences. To clarify the mechanisms of colonization and invasion of Af, we investigated the in vitro effects of Af culture filtrates (ACFs) on the functions of human alveolar macrophages (AMs), and polymorphonuclear leucocytes (PMNs). ACFs were obtained by culturing clinically isolated Af in Medium-199 at 37 degrees C for 5 days. In the study of phagocytosis of Af conidia by human AMs, 52% of AMs ingested conidia during a 60 min incubation period in Medium-199. However, the percentage decreased to 24% when incubated with a final concentration of 30% ACF in Medium-199. With respect to the antichemotactic activity on human PMNs, 3% ACF was sufficient for significant suppression, and 30% ACF completely inhibited the migration of PMNs. In addition, phorbol myristate acetate (PMA)-induced O2- release from PMNs was significantly suppressed in Medium-199 which included 12.5% ACF or more. The antichemotactic activity of ACF was partially abolished by trypsin or chicken egg ovomacroglobulin. When ACF was separated into two fractions (molecular weight > 10 and < 10 kDa) by dialysis and centrifugation through CL-LGC filters, both fractions retained the antichemotactic activity. We conclude that Af produce several antiphagocytic factors, which can be responsible for the colonization of Af in the bronchopulmonary tissues and allow this species to invade surrounding lung tissues in pulmonary aspergillosis by suppressing local host defences.


This article has been cited by other articles:


Home page
Eukaryot CellHome page
J. A. Sugui, J. Pardo, Y. C. Chang, A. Mullbacher, K. A. Zarember, E. M. Galvez, L. Brinster, P. Zerfas, J. I. Gallin, M. M. Simon, et al.
Role of laeA in the Regulation of alb1, gliP, Conidial Morphology, and Virulence in Aspergillus fumigatus
Eukaryot. Cell, September 1, 2007; 6(9): 1552 - 1561.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
J. Pardo, C. Urban, E. M. Galvez, P. G. Ekert, U. Muller, J. Kwon-Chung, M. Lobigs, A. Mullbacher, R. Wallich, C. Borner, et al.
The mitochondrial protein Bak is pivotal for gliotoxin-induced apoptosis and a critical host factor of Aspergillus fumigatus virulence in mice
J. Cell Biol., August 14, 2006; 174(4): 509 - 519.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
R. E. Lewis, N. P. Wiederhold, M. S. Lionakis, R. A. Prince, and D. P. Kontoyiannis
Frequency and Species Distribution of Gliotoxin-Producing Aspergillus Isolates Recovered from Patients at a Tertiary-Care Cancer Center
J. Clin. Microbiol., December 1, 2005; 43(12): 6120 - 6122.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
R. E. Lewis, N. P. Wiederhold, J. Chi, X. Y. Han, K. V. Komanduri, D. P. Kontoyiannis, and R. A. Prince
Detection of Gliotoxin in Experimental and Human Aspergillosis
Infect. Immun., January 1, 2005; 73(1): 635 - 637.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
B. Philippe, O. Ibrahim-Granet, M. C. Prevost, M. A. Gougerot-Pocidalo, M. Sanchez Perez, A. Van der Meeren, and J. P. Latge
Killing of Aspergillus fumigatus by Alveolar Macrophages Is Mediated by Reactive Oxidant Intermediates
Infect. Immun., June 1, 2003; 71(6): 3034 - 3042.
[Abstract] [Full Text] [PDF]


Home page
Clin. Microbiol. Rev.Home page
J.-P. Latge
Aspergillus fumigatus and Aspergillosis
Clin. Microbiol. Rev., April 1, 1999; 12(2): 310 - 350.
[Abstract] [Full Text] [PDF]


Home page
ThoraxHome page
T. Murayama, R. Amitani, Y. Ikegami, R. Kawanami, W. J. Lee, and R. Nawada
Effects of Aspergillus fumigatus culture filtrate on antifungal activity of human phagocytes in vitro
Thorax, November 1, 1998; 53(11): 975 - 978.
[Abstract] [Full Text]


Home page
NEJMHome page
R. L. Kradin and E. J. Mark
Case 32-1998- An 83-Year-Old Woman with Long-Standing Asthma and Rapidly Progressing Pneumonia
N. Engl. J. Med., October 22, 1998; 339(17): 1228 - 1236.
[Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the European Respiratory Society.