ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Permissions
Right arrowRequest Permissions
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ying, S.
Right arrow Articles by Kay, A.B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ying, S.
Right arrow Articles by Kay, A.B.
Eur Respir J 2003; 22:207-212
Copyright ©ERS Journals Ltd 2003


Cyclosporin A, apoptosis of BAL T-cells and expression of Bcl-2 inasthmatics

S. Ying1,2, L.N. Khan1, Q. Meng1,2, N.C. Barnes3 and A.B. Kay1

1 Dept of Allergy and Clinical Immunology, Faculty of Medicine, Imperial College London, National Heart & Lung Institute, 2 Division of Asthma, Allergy and Lung Biology, Guy's, King's and St Thomas' School of Medicine and 3 London Chest Hospital, London, UK

CORRESPONDENCE: A.B. Kay, Dept of Allergy and Clinical Immunology, Faculty of Medicine, Imperial College London, National Heart & Lung Institute, Dovehouse Street, London, SW3 6LY, UK. Fax: 44 20 73763138. E-mail: a.b.kay@ic.ac.uk

Keywords: apoptosis, asthma, bronchoalveolar lavage, cyclosporin A, cytokine

Received: October 29, 2002
Accepted February 20, 2003

This study was supported by the National Asthma Campaign, London, UK.

The late asthmatic reaction is characterised by elevated numbers of interleukin-4/interleukin-5/CD4-positive T-helper cells type 2 in bronchoalveolar lavage fluid (BALF). Cyclosporin A (CsA) is known to inhibit T-cell proliferation, induce apoptosis of CD4-positive T-cells and downregulate cytokine gene expression.

It was assessed whether CsA-induced inhibition of the late asthmatic reaction was associated with apoptosis of BALF T-lymphocytes and other cell types, as well as expression of the antiapoptotic protein B-cell leukaemia/lymphoma 2 gene product (Bcl-2). BALF cells were obtained from asthmatics at baseline and 24 h after allergen-inhalation challenge following prior administration of CsA (n=13) or placebo (n=11).

The number of apoptotic CD3-positive T-lymphocytes increased in the CsA but not the placebo group. The numbers of Bcl-2-positive cells were significantly reduced in the CsA but not the placebo group. The majority of Bcl-2-positive cells were CD3-positive T-lymphocytes.

The beneficial effect of cyclosporin A in asthma may be related to its inhibitory effect on the late asthmatic reaction via induction of T-cell apoptosis and decreased B-cell leukaemia/lymphoma 2 gene product levels.




This article has been cited by other articles:


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. Cheng, Z. Shao, B. Chaudhari, and D. K. Agrawal
Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1339 - L1347.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
M. Muller, J. Grunewald, C. Olgart Hoglund, B. Dahlen, A. Eklund, and H. Stridh
Altered apoptosis in bronchoalveolar lavage lymphocytes after allergen exposure of atopic asthmatic subjects
Eur. Respir. J., September 1, 2006; 28(3): 513 - 522.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
J. P. Lamb, A. James, N. Carroll, L. Siena, J. Elliot, and A. M. Vignola{dagger}
Reduced apoptosis of memory T-cells in the inner airway wall of mild and severe asthma
Eur. Respir. J., August 1, 2005; 26(2): 265 - 270.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the European Respiratory Society.