| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Original Articles |
Tight junctions (TJs) make a vital contribution to the barrier properties of the airway lining. Opening of TJs, or their frank cleavage, is suspected as a pathophysiological event in the lung, but research into the cellular and molecular mechanisms involved has been impeded by technical limitations of available experimental models. The authors have compared the properties of two epithelial cell lines derived from bronchial epithelium to explore whether these cell lines could constitute appropriate tools for the study of TJ regulation in bronchial epithelium. Investigations of TJs in 16HBE14o- cells and Calu-3 cells were made by fluorescent antibody labelling in conjunction with wide-field, confocal or 2-photon molecular excitation microscopy (2PMEM). The presence of TJ proteins was confirmed by immunoblotting and functional properties of the monolayers were studied by measurements of transepithelial electrical resistance and mannitol permeability. Cells of both lines formed confluent monolayers in which the cells expressed the TJ proteins occludin and ZO-1 in continuous circumferential patterns suggestive of functional TJs. This interpretation was supported by the development of transepithelial electrical resistances and of low paracellular permeability to solutes. Within the limits of resolution offered by 2PMEM, occludin and ZO-1 appeared to colocalize at TJs. These studies suggest that the 16HBE14o- cells and Calu-3 cell lines are potentially useful in vitro models to study how tight junction opening or cleavage changes the functional barrier properties of bronchial epithelium.
This article has been cited by other articles:
|
|
 |
|
  C. E. Olsen, A. E. Liguori, Y. Zong, R. C. Lantz, J. L. Burgess, and S. Boitano Arsenic upregulates MMP-9 and inhibits wound repair in human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L293 - L302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tahara, M. Takeda, Y. Shirogane, T. Hashiguchi, S. Ohno, and Y. Yanagi Measles Virus Infects both Polarized Epithelial and Immune Cells by Using Distinctive Receptor-Binding Sites on Its Hemagglutinin J. Virol., May 1, 2008; 82(9): 4630 - 4637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu and S. Chu ENaC {alpha}-subunit variants are expressed in lung epithelial cells and are suppressed by oxidative stress Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1454 - L1462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Grab, E. Nyarko, N. C. Barat, O. V. Nikolskaia, and J. S. Dumler Anaplasma phagocytophilum-Borrelia burgdorferi Coinfection Enhances Chemokine, Cytokine, and Matrix Metalloprotease Expression by Human Brain Microvascular Endothelial Cells Clin. Vaccine Immunol., November 1, 2007; 14(11): 1420 - 1424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Schnackenberg, S. M. Jones, C. Pate, B. Shank, L. Sessions, L. M. Pittman, L. E. Cornett, and R. C. Kurten The beta-agonist isoproterenol attenuates EGF-stimulated wound closure in human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L485 - L491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Kim, U. S. Sajjan, G. P. Krasan, and J. J. LiPuma Disruption of Tight Junctions during Traversal of the Respiratory Epithelium by Burkholderia cenocepacia Infect. Immun., November 1, 2005; 73(11): 7107 - 7112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Polette, C. Gilles, B. Nawrocki-Raby, J. Lohi, W. Hunziker, J.-M. Foidart, and P. Birembaut Membrane-Type 1 Matrix Metalloproteinase Expression Is Regulated by Zonula Occludens-1 in Human Breast Cancer Cells Cancer Res., September 1, 2005; 65(17): 7691 - 7698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Lafontaine, D. Wall, S. L. Vanlerberg, H. Donabedian, and D. D. Sledjeski Moraxella catarrhalis Coaggregates with Streptococcus pyogenes and Modulates Interactions of S. pyogenes with Human Epithelial Cells Infect. Immun., November 1, 2004; 72(11): 6689 - 6693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. A. Darling and T. J. Evans Effects of Nitric Oxide on Pseudomonas aeruginosa Infection of Epithelial Cells from a Human Respiratory Cell Line Derived from a Patient with Cystic Fibrosis Infect. Immun., May 1, 2003; 71(5): 2341 - 2349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Tucker, K. Varga, Z. Bebok, A. Zsembery, N. A. McCarty, J. F. Collawn, E. M. Schwiebert, and L. M. Schwiebert Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids Am J Physiol Cell Physiol, March 1, 2003; 284(3): C791 - C804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Asokananthan, P. T. Graham, D. J. Stewart, A. J. Bakker, K. A. Eidne, P. J. Thompson, and G. A. Stewart House Dust Mite Allergens Induce Proinflammatory Cytokines from Respiratory Epithelial Cells: The Cysteine Protease Allergen, Der p 1, Activates Protease-Activated Receptor (PAR)-2 and Inactivates PAR-1 J. Immunol., October 15, 2002; 169(8): 4572 - 4578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ahdieh, T. Vandenbos, and A. Youakim Lung epithelial barrier function and wound healing are decreased by IL-4 and IL-13 and enhanced by IFN-gamma Am J Physiol Cell Physiol, December 1, 2001; 281(6): C2029 - C2038. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
