| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Original Articles |
Human neutrophils contain three isoforms of antimicrobial and cytotoxic peptides in the azurophil granules, which belong to a family of mammalian neutrophil peptides named defensins. Here we investigate the role of these peptides in diffuse panbronchiolitis (DPB). Defensins (human neutrophil peptide-1, -2 and -3) were measured by radioimmunoassay in bronchoalveolar lavage fluid (BALF) of 30 patients with DPB, 16 patients with idiopathic pulmonary fibrosis (IPF) and 15 healthy adults. The concentration of defensins was higher in BALF of patients with DPB than in patients with IPF and healthy subjects. DPB and IPF patients also had significantly higher plasma concentrations of defensins than controls. In patients with DPB, BALF concentration of defensins correlated significantly with neutrophil count or BALF concentration of interleukin (IL)-8. Immunohistochemistry of open-lung biopsy specimens from four DPB patients showed localization of defensins in neutrophils and mucinous exudate in the airways, and on the surface of bronchiolar epithelial cells. In vitro studies showed an enhanced extracellular release of defensins following stimulation of neutrophils with phorbol myristate acetate, N-formyl-methionyl-leucyl-phenyalamine, and human recombinant IL-8. Treatment of DPB with macrolides for 6 months significantly reduced neutrophil count and concentrations of defensins and IL-8 in BALF. Our results indicate accumulation of neutrophil-derived defensins in the airway in diffuse panbronchiolitis, and suggest that defensins may be a marker of neutrophil activity in this disease.
This article has been cited by other articles:
|
|
 |
|
  H. Ishimoto, H. Mukae, N. Sakamoto, M. Amenomori, T. Kitazaki, Y. Imamura, H. Fujita, H. Ishii, S. Nakayama, K. Yanagihara, et al. Different effects of telithromycin on MUC5AC production induced by human neutrophil peptide-1 or lipopolysaccharide in NCI-H292 cells compared with azithromycin and clarithromycin J. Antimicrob. Chemother., January 1, 2009; 63(1): 109 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. McMorran, S. A. Ouvry Patat, J. B. Carlin, K. Grimwood, A. Jones, D. S. Armstrong, J. C. Galati, P. J. Cooper, C. A. Byrnes, P. W. Francis, et al. Novel Neutrophil-Derived Proteins in Bronchoalveolar Lavage Fluid Indicate an Exaggerated Inflammatory Response in Pediatric Cystic Fibrosis Patients Clin. Chem., October 1, 2007; 53(10): 1782 - 1791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Khine, L. Del Sorbo, R. Vaschetto, S. Voglis, E. Tullis, A. S. Slutsky, G. P. Downey, and H. Zhang Human neutrophil peptides induce interleukin-8 production through the P2Y6 signaling pathway Blood, April 1, 2006; 107(7): 2936 - 2942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ishimoto, H. Mukae, Y. Date, T. Shimbara, M. S. Mondal, J. Ashitani, T. Hiratsuka, S. Kubo, S. Kohno, and M. Nakazato Identification of hBD-3 in respiratory tract and serum: the increase in pneumonia Eur. Respir. J., February 1, 2006; 27(2): 253 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sakamoto, H. Mukae, T. Fujii, H. Ishii, S. Yoshioka, T. Kakugawa, K. Sugiyama, Y. Mizuta, J.-i. Kadota, M. Nakazato, et al. Differential effects of {alpha}- and {beta}-defensin on cytokine production by cultured human bronchial epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L508 - L513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Schultz Macrolide activities beyond their antimicrobial effects: macrolides in diffuse panbronchiolitis and cystic fibrosis J. Antimicrob. Chemother., July 1, 2004; 54(1): 21 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. T. Spencer, G. Paone, P. M. Krein, F. N. Rouhani, J. Rivera-Nieves, and M. L. Brantly Role of human neutrophil peptides in lung inflammation associated with {alpha}1-antitrypsin deficiency Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L514 - L520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bals and P.S. Hiemstra Innate immunity in the lung: how epithelial cells fight against respiratory pathogens Eur. Respir. J., February 1, 2004; 23(2): 327 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Rubin and M. O. Henke Immunomodulatory Activity and Effectiveness of Macrolides in Chronic Airway Disease Chest, February 1, 2004; 125(2_suppl): 70S - 78S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Hiratsuka, H Mukae, H Iiboshi, J Ashitani, K Nabeshima, T Minematsu, N Chino, T Ihi, S Kohno, and M Nakazato Increased concentrations of human {beta}-defensins in plasma and bronchoalveolar lavage fluid of patients with diffuse panbronchiolitis Thorax, May 1, 2003; 58(5): 425 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Aarbiou, M. Ertmann, S. van Wetering, P. van Noort, D. Rook, K. F. Rabe, S. V. Litvinov, J. H. J. M. van Krieken, W. I. de Boer, and P. S. Hiemstra Human neutrophil defensins induce lung epithelial cell proliferation in vitro J. Leukoc. Biol., July 1, 2002; 72(1): 167 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Mukae, H Iiboshi, M Nakazato, T Hiratsuka, M Tokojima, K Abe, J Ashitani, J Kadota, S Matsukura, and S Kohno Raised plasma concentrations of {alpha}-defensins in patients with idiopathic pulmonary fibrosis Thorax, July 1, 2002; 57(7): 623 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Paone, A. Wada, L. A. Stevens, A. Matin, T. Hirayama, R. L. Levine, and J. Moss ADP ribosylation of human neutrophil peptide-1 regulates its biological properties PNAS, June 11, 2002; 99(12): 8231 - 8235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Ashitani, H. Mukae, T. Hiratsuka, M. Nakazato, K. Kumamoto, and S. Matsukura Elevated Levels of {alpha}-Defensins in Plasma and BAL Fluid of Patients With Active Pulmonary Tuberculosis Chest, February 1, 2002; 121(2): 519 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Klut, B. A. Whalen, and J. C. Hogg Dynamic Changes in Neutrophil Defensins during Endotoxemia Infect. Immun., December 1, 2001; 69(12): 7793 - 7799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Ashitani, H. Mukae, T. Hiratsuka, M. Nakazato, K. Kumamoto, and S. Matsukura Plasma and BAL Fluid Concentrations of Antimicrobial Peptides in Patients With Mycobacterium avium- intracellulare Infection Chest, April 1, 2001; 119(4): 1131 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. van Wetering, A. C. van der Linden, M. A. J. A. van Sterkenburg, W. I. de Boer, A. L. A. Kuijpers, J. Schalkwijk, and P. S. Hiemstra Regulation of SLPI and elafin release from bronchial epithelial cells by neutrophil defensins Am J Physiol Lung Cell Mol Physiol, January 1, 2000; 278(1): L51 - L58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Teramoto, T. Mastsue, Y. Ouchi, and B. K. Rubin Manipulation of Volume vs Osmolality in Cystic Fibrosis Lung Disease Chest, November 1, 1999; 116(5): 1494 - 1495. [Full Text] [PDF]
|
|
|
|
 |
|
 B. AGERBERTH, J. GRUNEWALD, E. CASTAÑOS-VELEZ, B. OLSSON, H. JÖRNVALL, H. WIGZELL, A. EKLUND, and G. H. GUDMUNDSSON Antibacterial Components in Bronchoalveolar Lavage Fluid from Healthy Individuals and Sarcoidosis Patients Am. J. Respir. Crit. Care Med., July 1, 1999; 160(1): 283 - 290. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
